Author/Authors :
Ghanadian، M. نويسنده Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, I.R.Iran . , , Ayatollahi، A.M. نويسنده Phytochemistry Research Center & School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, I.R. Iran. , , Mesaik، M.A. نويسنده Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan. , , Afsharypour، S. نويسنده Department of Pharmacognosy and Isfahan Pharmaceutical Science Research Center, School of Pharmacy and Pharmaceutical Science, Isfahan University of Medical Science,, Isfahan, I.R.Iran. , , Abdalla، O.M. نويسنده Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan. , , Kobarfard، F. نويسنده ,
Abstract :
The cytotoxic chloroform fraction of Euphorbia aellenii Rech. F. (Euphorbiaceae) afforded two new phorbol diterpenoids: 4-deoxy-4?-phorbol-12-(2,3-dimethyl) butyrate-13-isobutyrate and 17-hydroxy-4-deoxy-4?-phorbol-12-(2,3-dimethyl) butyrate-13-isobutyrate. Their structures were elucidated by NMR and other spectroscopic methods. The immunomodulating potentials of the isolated compounds were tested using standard proliferation and chemiluminescence assays. Compound 2 showed moderate inhibitory activity against both T-cell proliferation and reactive oxygen species (ROS) production in whole blood with IC50 of 14.0 ± 0.57 and 44.1 ± 3.8 ?g/ml, respectively, while compound 1 was relatively inactive with IC50 > 50 ?g/mL for T-cell proliferation, and > 100 ?g/mL for ROS.
