Author/Authors :
Abdelrhman، Salah Shaban نويسنده Animal Resources Adm., Ministry of Agriculture 11195, Riyadh, Kingdom of Saudi Arabia Abdelrhman, Salah Shaban , M. Rihan، Ehab Abd-ELsabour نويسنده Veterinary Diagnostic Laboratory, Ministry of Agriculture, Riyadh 11454, Saudi Arabia M. Rihan, Ehab Abd-ELsabour , Almotairy، Hany M نويسنده King AbdelAziz City for Science and Technology, Riyadh, Saudi Arabia Almotairy, Hany M , Al Jassem، Ali H نويسنده Animal Resources Adm., Ministry of Agriculture 11195, Riyadh, Kingdom of Saudi Arabia Al Jassem, Ali H , Al-Blowi، M H نويسنده Animal Resources Adm., Ministry of Agriculture 11195, Riyadh, Kingdom of Saudi Arabia Al-Blowi, M H
Abstract :
Two hundred and sixteen samples (80 Tracheal swabs and 136 blood serum) were collected from four commercial ostrich farms in the Kingdom of Saudi Arabia for isolation of Newcastle disease (ND) and Avian Influenza (AI) viruses or detection of their antigens and antibodies by RT-PCR and Enzyme Linked Immune Sorbant Assay (ELISA) respectively. Neither AI and ND viruses nor their antigens were isolated or detected in any of the collected swabs samples. Also, Antibodies to avian influenza virus were not detected in the collected serum samples. Antibodies to ND virus were detected in 0.0%, 80.0%, 74.1%, and 80.0% in serum samples from farms 1, 2, 3 and 4, respectively. Mean ND ELISA antibody titer were 0.0, 646.3, 1068.5 and 1440.5 in farms 1, 2, 3 and 4, respectively. Ostriches vaccinated with live ND vaccine by drinking water were negative for ND antibodies, while ostriches vaccinated with live ND vaccine by spray method and boosted by inactivated oil emulsion ND vaccine gave the highest immune response. Ostriches vaccinated with inactivated oil emulsion ND vaccine (4x chicken dose/ostrich) elicited effective immune response and transferred maternal antibodies to ostrich chicks at the rate of 85.7% and 66.7% with mean ELISA antibody titer of 1450.5 and 1350.15 at 7 and 21 day old, respectively.
