Author/Authors :
Olga V. Chervyakova، نويسنده , , Vitaliy M. Strochkov، نويسنده , , Elmira T. Tailakova، نويسنده , , Kulyaisan T. Sultankulova، نويسنده , , Nurlan T. Sandybayev، نويسنده , , Abylay R. Sansyzbay، نويسنده , , Nikolay E. Gorev، نويسنده , , Maria V. Sergeeva، نويسنده , , Marina V. Potapchuk، نويسنده , , Irina A. Repko، نويسنده , , Ludmila M. Tsybalova، نويسنده , , Oleg I. Kiselev، نويسنده ,
Abstract :
Recombinant cold-adapted strain A/HK/Otar/6:2/2010 (H3N8) constructed by the method of classic genetic reassortment, inherited the hemagglutinin and neuraminidase genes from the epizootic A/equine/Otar/764/2007 (Н3N8) virus and genes of nonglycosylated proteins from attenuation donor A/Hong Kong/1/68/162/35 (H3N2). The resulted virus was of cold-adapted (efficient growth at 25°C) and temperature-sensitive (restricted replication at 39°C) phenotype. Like the parental virus of subtype H3N2, the resulted reassortant was attenuated, with limited replication in lungs of mice (1.75 lgEID50/mL) versus replication in turbinate (2.5 lgEID50/mL). Intranasal immunization of mice with A/HK/Otar/6:2/2010 (H3N8) reassortant did not induce animal weight reduction in contrast to epizootic A/equine/Otar/764/2007 (Н3N8) virus.
