Author/Authors :
Doranie uliaie، Ebrahim نويسنده Department of Plant Breeding and Biotechnology, Tabriz University , , Ghareyazi، Behzad نويسنده , , Farsi ، Mohammad نويسنده Faculty of Chemical and Petroleum Engineering, Shiraz University,Shiraz, I.R. IRAN , , Kogel، Karl-Heinz نويسنده , , Imani، Jafar نويسنده Research Centre for BioSystems, Land Use and Nutrition (IFZ), Justus Liebig University, Giessen, Germany. ,
Abstract :
A significant portion of the world’s cultivated land is affected by salinity that reduces crop productivity in
these areas. Breeding for salt tolerance is one of the important strategies to overcome this problem. Recently,
genetic engineering is becoming a promising approach to improving salt tolerance. In order to improve the
yield performance of canola in saline soils, we transformed canola with Arabidopsis vacuolar Na+/H+
antiporter gene AtNHX1 which enhances the plant capacity for reducing cytosolic Na+ by transporting Na+
into the vacuole. Southern analysis of putative transgenic plants indicated that only one copy of the gene
integrated into the plant genome. Overexpression of the AtNHX1 gene was shown in T1 transgenic plants.
Under salinity conditions, stem and root length and overall biomass of transgenic plants were significantly
higher compared to those of nontransgenic plants. Moreover, salt treated transgenic plants contained high
proline and K+, but less Na+ compared to wild type.
