Author/Authors :
BARKHORDARI، ABOLFAZL نويسنده Department of Occupational Health, School of Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , , Hekmatimoghaddam، Seyedhossein نويسنده Department of Laboratory Sciences, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , , Jebali، Ali نويسنده Department of Medical Nanotechnology, Pajoohesh Lab, Yazd, Iran. , , Khalili، Mohammad Ali نويسنده Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , , Talebi، Alireza نويسنده Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , , Noorani، Marzieh نويسنده Department of Occupational Health, School of Paramedicine, Abarkooh, Yazd, Iran ,
Abstract :
Background: The extensive use of different nanoparticles has raised great concerns about their occupational and biological safety.
Objective: The aim of this study was to evaluate the cytotoxic effect of zinc oxide nanoparticles (ZnO NPs) on viability of spermatozoa.
Materials and Methods: Semen samples were obtained from 15 healthy persons, and were analyzed using WHO guidelines. Each semen sample was separately incubated with different concentrations of ZnO NPs (10, 100, 500, and 1000 ?g/mL) at 37PoPC for 45, 90, and 180 minutes. Then, the cell death percentage of spermatozoa was measured by MTT assay. Mann-Whitney test was used for comparison of different times and concentrations.
Results: The maximum cell death percentage was 20.8%, 21.2%, and 33.2% after 45, 90, and 180 minutes, respectively. In case of concentration, the highest concentration (1000 ?g/mL) of ZnO NPs led to the highest toxicity for all incubation times. Statistically, there were significant differences in cell viability after 180 minutes vs. 45 and 90 minutes.
Conclusion: This study indicated that cytotoxicity of ZnO NPs is dose and time dependent
