Author/Authors :
Reshma Anjum، M نويسنده Department of Biotechnology, S.V. University, Tirupati-517502 , , Hari Priya، p نويسنده Department of Biotechnology, S.V. University, Tirupati-517502 , , Madhu، P نويسنده Department of Biotechnology, S.V. University, Tirupati-517502 , , Pratap Reddy، K نويسنده Department of Biotechnology, S.V. University, Tirupati-517502 , , Sreenivasula Reddy، P نويسنده Department of Zoology, S.V. University, Tirupati-517502 ,
Abstract :
Lead or lead together with testosterone was given to adult rats in order to assess the protective effect of
testosterone supplementation on lead-induced suppressed fertility in male rats. Male Wistar rats were exposed to
lead acetate at dose level of 0.05 and 0.15 % for 55 days through drinking water and injected intraperitoneally with
either testoviron depot at a dose of 4.16 mg/Kg body weight or vehicle alone on day 1, 7 and 14. To assess the
fertility, control and experimental males were cohabited with sexually mature 100 day old females for 8 days.
Significant decrease in mating index and fertility index were observed with an increase in conception time with male
rats exposed to lead. Significant decrease was alsoobserved in fertility rate, measured by counting live fetuses in the
uterus of females mated with males exposed to lead.Pre- and post- implantation losses were increased significantly
in females mated with lead exposed males. Testosterone supplementation restored the suppressed fertility in Pb-exposed rats. These data demonstrate that fertilization capacity of adult male rats exposed to lead decreased
significantly and testosterone supplementation mitigated lead-induced suppressed male fertility.
