Author/Authors :
SS، Abdel Rhman نويسنده Animal Resources Administration, Ministry of Agriculture, Riyadh, 11195 Kingdom of Saudi Arabia SS, Abdel Rhman , AH، Al Jassem نويسنده Animal Resources Administration, Ministry of Agriculture, Riyadh, 11195 Kingdom of Saudi Arabia AH, Al Jassem , G M، Hussein نويسنده Veterinary Vaccine Production Center, Ministry of Agriculture Riyadh 11454, Saudi Arabia G M, Hussein , Al-Blowi، M H نويسنده Animal Resources Administration, Ministry of Agriculture, Riyadh, 11195 Kingdom of Saudi Arabia Al-Blowi, M H
Abstract :
Comparison between Haemagglutination Inhibition (HI) test and Enzyme Linked Immune Sorbent Assay (ELISA) in evaluation of humoral immune response to Newcastle disease (ND) in vaccinated quails was investigated. The obtained results showed higher NDV antibody titer (2, 5 and 8) log2 at zero, 14 and 28 day post vaccination in serum of La Sota vaccinated quails when the antigen used in HI test was homologous (La Sota), whereas lower antibody response (2, 4 and 6) log2 were obtained when the antigen used was heterologous (Hitchner B1). However, antibody titers in serum sample from Hitchner B1 vaccinated quails were (2, 4 and 6) log2 at zero, 14 and 28 day post vaccination against both homologous (Hitchner B1) and heterologous (La Sota) antigens. Non treated sera from control non vaccinated quails gave false positive results (6 log2) in HI test. Treatment of serum by heat (56´C /30 minutes) or by washing with 10% Chicken red blood overcome false positive results obtained by non treated serum in HI test. No difference between chicken and quail red blood cells were observed when used as red blood cells indicators in HI test to detect Newcastle disease virus antibodies of vaccinated quails. Two commercial ELISA kits used in this study failed to detect any positive sera (0.0 %) for ND antibodies in both vaccinated and non vaccinated quails at different time periods.
