Electrochemical detection of aberrant methylated gene using naphthalene diimide derivative carrying four ferrocene moieties

Naphthalene diimide derivative, F4ND, carrying four ferrocene moieties was synthesized as a new hybridization indicator for electrochemical gene detection using DNA probe-immobilized electrode. F4ND could bind to double stranded DNA with threading intercalation mode despite the existence of such bulky substituents. As result, an improved binding affinity due to slower dissociation from double stranded DNA was observed. F4ND could be used for electrochemical discrimination between methylated and unmethylated PCR product of CDH4 gene coupled with methylation specific DNA probe-immobilized electrode under the optimum condition.