Specificity of Lysine:N6-Hydroxylase: A Hypothesis for a Reactive Substrate Intermediate in the Catalytic Mechanism

The recombinant cytoplasmic preparation of lysine:N6-hydroxlase catalyzes the conversion ofL-lysine to itsN6-hydroxy derivative when supplemented with the cofactors NADPH and FAD. A number of lysine analogs reflecting minor alterations in the inherent structural features of the amino acid as well compounds with relatively high affinity for lysine binding domains in other proteins were examined for their ability to serve as substrates of lysine:N6-hydroxylase. These studies have revealed that the enzyme does not tolerate any change in the structural features ofL-lysine, its preferred substrate, with the exception of the replacement of the CγH2-methylene group by sulfur, as in (S)-2-aminoethyl-L-cysteine.L-Norleucine is a potent inhibitor of the enzyme whileL-norvaline andL-α-aminobutyric acid do not exhibit such effect, indicating the importance of a C4hydrophobic side chain for effective interaction with the enzyme. Among theN-alkyl amides of hydrophobic amino acids, onlyL-norleucine methylamide andL-α-aminobutyric acid ethylamide serve as moderate inhibitors of lysine:N6-hydroxylase. Based on the enzymeʹs stringent substrate specificity, a mechanism involving the conversion ofL-lysine to 2-aminocaprolactam prior to its oxygenation by the 4a-peroxyflavin intermediate in the catalytic cycle is proposed.