Methyltrypsin-Catalyzed Peptide Coupling: Comparison of Alkyl Ester and Guanidinophenyl Ester Derivatives as Acyl Donor Component

Methyltrypsin-catalyzed peptide synthesis has been studied by using conventional alkyl ester andp-guanidinophenyl ester derivatives of α-amino acid as the acyl donor component. They were found to be coupled with α-amino acid derivatives (acyl acceptor component) to produce dipeptide. The behavior of methyltrypsin toward both the substrates has been studied.