Reliability of cardiorespiratory measurements with a new ergospirometer during intense treadmill exercise in Thoroughbred horses

This study investigated the reliability of measurements with a new equine ergospirometer (Quadflow). Heart rate and blood lactate responses during exercise in horses wearing the Quadflow and an open flow mask were also compared. The mean percentage error of the oxygen uptake measurements was 8.2% (range 2.1–12.5%). Percent error for peak expiratory flow rates ranged from 6.1% to 9.4 %, and for minute ventilation from 2.5% to 7.4%. The coefficients of variation of the means of four measurements in two horses exercising continuously at 9.0 m/s were <5% for variables related to pulmonary ventilation, and was 7.7% for oxygen uptake. The Quadflow mask resulted in small increases in blood lactate concentration and relative heart rate during submaximal exercise. It was concluded that between- and within-test reliability statistics for important measurements in equine clinical exercise testing were acceptable for routine use in a veterinary practice or research laboratory.