Author/Authors :
dos Santos، نويسنده , , Debora Regina Lopes and Vitral، نويسنده , , Claudia Lamarca and de Paula، نويسنده , , Vanessa Salete and Marchevsky، نويسنده , , Renato Sergio and Lopes، نويسنده , , Juliana Freitas and Gaspar، نويسنده , , Ana Maria Coimbra and Saddi، نويسنده , , Telma Michella and Jْnior، نويسنده , , Nilton Cecيlio de Mesquita and Guimarمes، نويسنده , , Flavio de Rezende and Jْnior، نويسنده , , Joمo Garcia Caramori and Ximenes، نويسنده , , Lia Laura Lewis and Souto، نويسنده , , Francisco José Dutra and Pinto، نويسنده , , Marcelo Alves، نويسنده ,
Abstract :
Active hepatitis E virus (HEV) infections in two Brazilian swine herds were investigated. In study 1, 26 piglets born to five anti-HEV positive sows were monitored from birth to post-partum week 22. Serum samples were screened for the detection of anti-HEV antibodies and a nested RT-PCR used to examine the HEV genome. Passive transfer of immunity was confirmed. At week 22, 23/26 (88.4%) of the piglets had seroconverted. Genome amplification was achieved in a feces pool from one holding pen and in one serum sample, both from 13-week-old animals. Histology was suggestive of a potential HEV infection. In the second study, 47 piglets born to six anti-HEV-positive sows were monitored after weaning. Seroconversion was determined in eight animals at 6–8 weeks of age. HEV RNA was detected in two pools from a holding pen for 12–16-week-old animals. Brazilian isolates were classified as genotype 3. This is the first molecular evidence of HEV infection in Brazilian pig herds.
Keywords :
Brazil , Swine , hepatitis E virus , molecular epidemiology
