Author/Authors :
Gutiérrez، نويسنده , , A.M. and Miller، نويسنده , , I. and Hummel، نويسنده , , K. and Nِbauer، نويسنده , , K. and Martيnez-Subiela، نويسنده , , S. and Razzazi-Fazeli، نويسنده , , E. and Gemeiner، نويسنده , , M. and Cerَn، نويسنده , , J.J.، نويسنده ,
Abstract :
Saliva contains a number of proteins that may be useful as biomarkers of health and disease and can be easily obtained from large numbers of animals in a non-invasive, stress-free way. The objective of this study was to explore the protein composition of porcine saliva from 10 specific pathogen free pigs using first one-dimensional SDS–PAGE and then two-dimensional electrophoresis and mass spectrometry. A reference proteome pattern for porcine saliva was established with the identification of 13 different, mainly saliva-specific, proteins. These reference data will facilitate the investigation of salivary proteins potentially altered in disease and could serve as novel diagnostic biomarkers.
Keywords :
Proteome , Saliva , Porcine , Two-dimensional electrophoresis , mass spectrometry , Immunoblotting , biomarkers
