Author/Authors :
Pisoni، نويسنده , , Giuliano and D’Amelio، نويسنده , , Patrizia and Sassi، نويسنده , , Francesca and Manarolla، نويسنده , , Giovanni and Scaccabarozzi، نويسنده , , Licia and Locatelli، نويسنده , , Clara and Mazzocchi، نويسنده , , Nausicaa and Baruscotti، نويسنده , , Mirko and DiFrancesco، نويسنده , , Dario and Moroni، نويسنده , , Paolo، نويسنده ,
Abstract :
Formation of multinucleated giant cells (MGCs) by macrophage fusion is a typical cytopathic effect of lentiviral replication in caprine monocytes and MGC formation from cultured caprine peripheral blood mononuclear cells (PBMCs) has been considered to be diagnostic for small ruminant lentivirus (SRLV) infection. In this study, formation of MGCs was observed after 7–14 days when PBMCs were cultured from healthy goats free from SRLV infection. These MGCs expressed tartrate-resistant acid phosphatase, calcitonin receptor, integrin αVβ3, cathepsin K and matrix metalloproteinase 9 and were able to resorb bone in vitro in the absence of RANKL and macrophage colony stimulating factor, consistent with an osteoclast phenotype.
Keywords :
peripheral blood mononuclear cells , Osteoclast , caprine , Multinucleated giant cells
