Author/Authors :
Knudsen، نويسنده , , Tom and Kjelgaard-Hansen، نويسنده , , Mads and Tranholm، نويسنده , , Mikael and Wiinberg، نويسنده , , Bo and Clausen، نويسنده , , Jes T. and Hansen، نويسنده , , Jens Jacob and Nichols، نويسنده , , Timothy C. and Kjalke، نويسنده , , Marianne and Jensen، نويسنده , , Asger L. and Kristensen، نويسنده , , Annemarie T.، نويسنده ,
Abstract :
Canine coagulation factor VII (FVII) deficiency can be hereditary or acquired and may cause life threatening bleeding episodes if untreated. FVII procoagulant activity can be measured by FVII activity (FVII:C), but assays for measurement of canine specific FVII antigen (FVII:Ag) have not been available to date. In this study, a canine specific ELISA for measurement of FVII:Ag in plasma was developed and validated. The FVII:Ag ELISA correctly diagnosed homozygous and heterozygous hereditary FVII deficiency. Together with activity based assays, such as FVII:C, the FVII:Ag ELISA should be valuable in the diagnosis of hereditary canine FVII deficiency.
Keywords :
Coagulation factor , ELISA , FVII deficiency , haemostasis , PLASMA , IMMUNOASSAYS
