Author/Authors :
Buishand، نويسنده , , Floryne O. and van Erp، نويسنده , , Mariëlle G.M. and Groenveld، نويسنده , , Harmke A. and Mol، نويسنده , , Jan A. and Kik، نويسنده , , Marja and Robben، نويسنده , , Joris H. and Kooistra، نويسنده , , Hans S. and Kirpensteijn، نويسنده , , Jolle، نويسنده ,
Abstract :
The long-term prognosis after surgical resection of canine insulinoma is poor. Signs of hypoglycaemia often recur soon after surgery because tumour tissue has only been resected partially and/or functional (micro-)metastases were present. Using quantitative real-time PCR, the expression of 16 target genes was compared between primary canine insulinomas and their corresponding metastases. There was significantly higher expression of genes encoding for growth hormone (GH) and insulin-like growth factor-1 (IGF-1) in metastases compared to their primary tumours. Immunohistochemical examination of proteins of the GH–IGF-1 axis revealed expression of GH, IGF-1 and GH receptor (GHR) in both primary insulinomas and metastases. Immunohistochemical staining for IGF-1 was significantly higher in metastases compared to primary tumours.
Keywords :
canine , ?-cell tumour , Growth hormone , Insulinoma , Insulin-Like Growth Factor
