Author/Authors :
Leon، نويسنده , , Priscila Marques Moura de and Campos، نويسنده , , Vinicus Farias and Thurow، نويسنده , , Helena Strelow and Hartwig، نويسنده , , Fernando Pires and Selau، نويسنده , , Lisiane Priscila and Dellagostin، نويسنده , , Odir Antonio and Neto، نويسنده , , José Braccini and Deschamps، نويسنده , , Joمo Carlos and Seixas، نويسنده , , Fabiana Kِmmling and Collares، نويسنده , , Tiago، نويسنده ,
Abstract :
Single nucleotide polymorphisms (SNPs) in the p53 gene have been studied extensively in humans. The aims of this study were to determine the frequency of the Arg/Pro SNP in p53 in Thoroughbred mares on one stud in Brazil and to correlate p53 genotypes with reproductive performance. SNPs were detected by PCR-restriction fragment length polymorphism in blood samples from 105 horses and confirmed by sequencing. The allele frequency in Thoroughbred mares at codon 72 in exon 4 was 73.3% Arg/Pro, 17.1% Arg/Arg and 9.6% Pro/Pro. The presence of Arg/Pro was significantly associated with abortion (P = 0.02), while Pro/Pro mares had a lower probability of abortion (P < 0.05). Using a logistic regression model, the dominance effect was significant (P = 0.044; odds ratio 7.94) for abortion and additive effects were not significant (P = 0.26). p53 may play a role in equine reproduction.
Keywords :
abortion , Thoroughbred , p53 , genetic polymorphism , equine
