Author/Authors :
Ochiai، نويسنده , , Kazuhiko and Watanabe، نويسنده , , Masami and Azakami، نويسنده , , Daigo and Michishita، نويسنده , , Masaki and Yoshikawa، نويسنده , , Yasunaga and Udagawa، نويسنده , , Chihiro and Metheenukul، نويسنده , , Pornphimon and Chahomchuen، نويسنده , , Thippayarat and Aoki، نويسنده , , Hiroshi and Kumon، نويسنده , , Hiromi and Morimatsu، نويسنده , , Masami and Omi، نويسنده , , Toshinori، نويسنده ,
Abstract :
REIC/Dkk-3, a member of the human Dickkopf (Dkk) family, plays a role as a suppressor of growth in several human cancers. In this study, the tumour suppression function of canine REIC/Dkk-3 was investigated. The full-length open reading frame of the canine REIC/Dkk-3 homologue was cloned and the tissue distribution of REIC/Dkk-3 mRNA was determined, along with the subcellular localisation of the REIC/Dkk-3 protein in canine cancer cell lines. Expression of REIC/Dkk-3 was lower in mammary gland tumours and in canine mammary carcinoma cell lines than in normal mammary gland tissue. Overexpression of REIC/Dkk-3 induced apoptosis in canine mammary carcinoma cell lines. These results show that expression of REIC/Dkk-3 is downregulated in canine mammary tumours and that one of the functions of this gene is induction of apoptosis.
Keywords :
Mammary gland tumours , REIC/Dkk-3 , canine , Tumour suppressor gene
