Agreement between the cell culture titrations of canine minute virus determined by two susceptibility-testing methods

The correct diagnosis of canine minute virus is critical in dog breeding. In this study, the Bland Altman test was used to compare the performance of two susceptibility-testing methods, namely polymerase chain reaction (PCR) and indirect immunofluorescence assay (IFA). The agreement between IFA and PCR in monocytes revealed a mean difference of −1752.16 with 95% confidence and an interval ranging from −3229.80 to −274.53 (SD = 2325.62). The agreement between IFA and PCR in Walter Reed canine cells (WRCC) revealed a mean difference of −2396.55 with 95% confidence and an interval ranging from −3774.63 to −1018.48 (SD = 2168.93). The Bland Altman test confirmed the overall accuracy of PCR vs IFA and the plot showed that all points were not randomly arranged in the range of average ±1.96 × SD of the differences.