A new tool for superoxide and nitric oxide radicals determination using suitable enzymatic sensors

A new tool for radical determination is investigated. Superoxide and nitric oxide radicals have been determined using their modulating action on three different enzymatic sensors based on tyrosinase, superoxide dismutase (SOD), and galactose oxidase (GAO), respectively. Basic research was carried out to study the inhibition or activation effect of the two radicals considered on the enzyme sensorʹs response and experimentally evidencing the correlation between the modulating action of the biosensor response and the radical concentration in solution. Results are shown as linear ranges, which differ according to the biosensor used and the radical determined.