Title of article :
Localization of injected apolipophorin III in vivo — new insights into the immune activation process directed by this protein
Author/Authors :
Marv Dettloff، نويسنده , , Matthias and Kaiser، نويسنده , , Brigitta and Wiesner، نويسنده , , Andreas، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2001
Pages :
9
From page :
789
To page :
797
Abstract :
A few years ago, it was shown that intrahemocoelic injection of the insect apolipoprotein apolipophorin III (apoLp-III) stimulates an immune response in larvae of the greater wax moth, Galleria mellonella. Since the mode of action of this activation process is unknown, we followed apoLp-IIIʹs pathway in the early phase of the immune-stimulating process, using biotin as a probe. Biotinylated apoLp-III was injected and localized using avidin-coupled horseradish peroxidase. The labeled protein was fully functional; the added amount of biotin per apoLp-III molecule used in this study only slightly decreased its ability to associate with phospholipase C-treated human low-density lipoprotein, as well as the immune-stimulating capability of apoLp-III. ectrophoresis with subsequent staining of biotin moieties and lipids revealed that apoLp-III undergoes lipid association in vivo within the first few minutes after injection. After two hours, no biotinylated apoLp-III was detectable in cell-free hemolymph. At this time, a subpopulation of hemocytes showed a distinct peroxidase staining. Control injections of biotinylated bovine serum albumin did not lead to similar results, giving evidence for the specificity of the phenomena observed. The results indicate that lipid association of apoLp-III occurs prior to endocytosis by immune-competent hemocytes, which is followed by the induction of a humoral immune response.
Keywords :
Hemocyte , Lipoprotein , Galleria mellonella , apolipophorin III , insect immunity
Journal title :
Journal of Insect Physiology
Serial Year :
2001
Journal title :
Journal of Insect Physiology
Record number :
1412241
Link To Document :
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