Assembling of recombinant calmodulin with modulating function on solid surface

Many types of proteins express their functions with conformation change in various biosystems. For the construction of biosensing system consisting of such functional proteins, the development of techniques for assembling of proteins with retaining their functions even on a solid substrate is required. Calmodulin was selected as a model protein for molecular assembly. Calmodulin expresses modulating function by conformation change with calcium binding. In the present study, some cysteine residues were introduced at the N-terminus of calmodulin by genetic engineering, and the resulting engineered calmodulin could be assembled efficiently with oriented manner. The modulating function of calmodulin on a solid-phase surface was also demonstrated.