Title of article :
RNA interference in Lepidoptera: An overview of successful and unsuccessful studies and implications for experimental design
Author/Authors :
Terenius، نويسنده , , Olle and Papanicolaou، نويسنده , , Alexie and Garbutt، نويسنده , , Jennie S. and Eleftherianos، نويسنده , , Ioannis and Huvenne، نويسنده , , Hanneke and Kanginakudru، نويسنده , , Sriramana and Albrechtsen، نويسنده , , Merete and An، نويسنده , , Chunju and Aymeric، نويسنده , , Jean-Luc and Barthel، نويسنده , , Andrea and Bebas، نويسنده , , Piotr and Bitra، نويسنده , , Kavita and Bravo، نويسنده , , Alejandra and Chevalier، نويسنده , , François and Collinge، نويسنده , , Derek P. and Crava، نويسنده , , Cristina M. and de Maagd، نويسنده , , Ruud A. and Duvic، نويسنده , , Bernard and Erlandson، نويسنده , , Martin and Faye، نويسنده , , Ingrid and Felfِldi، نويسنده , , Gabriella and Fujiwara، نويسنده , , Haruhiko and Futahashi، نويسنده , , Ryo and Gandhe، نويسنده , , Archana S. and Gatehouse، نويسنده , , Heather S. and Gatehouse، نويسنده , , Laurence N. and Giebultowicz، نويسنده , , Jadwiga M. and Gَmez، نويسنده , , Isabel and Grimmelikhuijzen، نويسنده , , Cornelis J.P. and Groot، نويسنده , , Astrid T. and Hauser، نويسنده , , Frank and Heckel، نويسنده , , David G. and Hegedus، نويسنده , , Dwayne D. and Hrycaj، نويسنده , , Steven and Huang، نويسنده , , Lihua and Hull، نويسنده , , J. Joe and Iatrou، نويسنده , , Kostas and Iga، نويسنده , , Masatoshi and Kanost، نويسنده , , Michael R. and Kotwica، نويسنده , , Joanna and Li، نويسنده , , Changyou and Li، نويسنده , , Jianghong and Liu، نويسنده , , Jisheng and Lundmark، نويسنده , , Magnus and Matsumoto، نويسنده , , Shogo and Meyering-Vos، نويسنده , , Martina and Millichap، نويسنده , , Peter J. and Monteiro، نويسنده , , Antَnia and Mrinal، نويسنده , , Nirotpal and Niimi، نويسنده , , Teruyuki and Nowara، نويسنده , , Daniela and Ohnishi، نويسنده , , Atsushi and Oostra، نويسنده , , Vicencio and Ozaki، نويسنده , , Katsuhisa and Papakonstantinou، نويسنده , , Maria and Popadic، نويسنده , , Aleksandar and Rajam، نويسنده , , Manchikatla V. and Saenko، نويسنده , , Suzanne and Simpson، نويسنده , , Robert M. and Soberَn، نويسنده , , Mario and Strand، نويسنده , , Michael R. and Tomita، نويسنده , , Shuichiro and Toprak، نويسنده , , Umut and Wang، نويسنده , , Ping and Wee، نويسنده , , Choon Wei and Whyard، نويسنده , , Steven and Zhang، نويسنده , , Wenqing and Nagaraju، نويسنده , , Javaregowda and ffrench-Constant، نويسنده , , Richard H. and Herrero، نويسنده , , Salvador and Gordon، نويسنده , , Karl and Swevers، نويسنده , , Luc and Smagghe، نويسنده , , Guy، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2011
Pages :
15
From page :
231
To page :
245
Abstract :
Gene silencing through RNA interference (RNAi) has revolutionized the study of gene function, particularly in non-model insects. However, in Lepidoptera (moths and butterflies) RNAi has many times proven to be difficult to achieve. Most of the negative results have been anecdotal and the positive experiments have not been collected in such a way that they are possible to analyze. In this review, we have collected detailed data from more than 150 experiments including all to date published and many unpublished experiments. Despite a large variation in the data, trends that are found are that RNAi is particularly successful in the family Saturniidae and in genes involved in immunity. On the contrary, gene expression in epidermal tissues seems to be most difficult to silence. In addition, gene silencing by feeding dsRNA requires high concentrations for success. Possible causes for the variability of success in RNAi experiments in Lepidoptera are discussed. The review also points to a need to further investigate the mechanism of RNAi in lepidopteran insects and its possible connection to the innate immune response. Our general understanding of RNAi in Lepidoptera will be further aided in the future as our public database at http://insectacentral.org/RNAi will continue to gather information on RNAi experiments.
Keywords :
Lepidoptera , Delivery methods , Tissue uptake , dsRNA properties , gene function , RNA interference
Journal title :
Journal of Insect Physiology
Serial Year :
2011
Journal title :
Journal of Insect Physiology
Record number :
1416135
Link To Document :
بازگشت