Title of article :
Study of pure urease Langmuir–Blodgett film and application for biosensor development
Author/Authors :
Hou، نويسنده , , Yanxia and Jaffrezic-Renault، نويسنده , , Nicole and Zhang، نويسنده , , Aidong and Wan، نويسنده , , Jialiang and Errachid، نويسنده , , Abdelhamid and Chovelon، نويسنده , , Jean-Marc، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2002
Abstract :
The formation of urease monolayer at air–water interface, Π–A isotherm and stability of this monolayer are studied as a function of urease concentration and pH of subphase. The optimal conditions for transfer of the Langmuir–Blodgett film (LB film) of urease onto the hydrophobic surface of octadecylsilane-modified SiO2/Si substrate are determined. The sensitivity of detection of urea of the enzyme/insulator/semiconductor (EnIS) system is found to be 22 mV/pUrea.
Keywords :
Urease , Langmuir–Blodgett films , Urea biosensor , Urease monolayer
Journal title :
Sensors and Actuators B: Chemical
Journal title :
Sensors and Actuators B: Chemical
