Immobilisation of bioreceptors for microreactors

In this paper, immobilisation techniques of bioreceptors for microsystems are presented. A method of silicon surface modification for biomolecules immobilisation under mild conditions was analysed. The method is based on the alkilation reaction between amino groups of the biomolecule and epoxy terminal on the surface. For chemical immobilisation of the bioreceptors, glycidoxypropylethoxysilane and 3-glycidoxypropylmethoxysilane were used. Evaluation of the immobilisation procedures was performed in a flow system.