Spectra analysis of interaction between poly((2-methylacryloxyethyl) dimethyl buthylammonium bromide) and nucleic acids

The interaction between poly((2-methylacryloxyethyl) dimethyl buthylammonium bromide) (PMDB) and DNA is studied by spectral method. The results show that it is a good way to determine 0.2–11.5 μg mL−1 DNA with PMDB by light scattering method. This method is reliable, precise and simple. FT-IR spectra and UV-spectra are used to study the mechanisms of the interaction. The results indicate that the conformation of the DNA has changed during the interaction because the microenvironment of DNA changes.