A disposable glucose biosensor based on diffusional mediator dispersed in nanoparticulate membrane on screen-printed carbon electrode

In this paper, we described a disposable glucose biosensor based on co-dispersion of a diffusional polymeric mediator, poly(vinylferrocene-co-acrylamide) (PVFcAA), and glucose oxidase (GOX) in alumina nanoparticulate membrane on screen-printed carbon electrode. The biosensor exhibited classical features of a kinetically fast redox couple. At scan rates up to 200 mV/s, the peak-to-peak potential separation of the mediator was 59 mV at 25 °C. In the presence of glucose, a typical catalytic oxidation current was observed, which reached a plateau at 0.30 V (versus Ag/AgCl). Under optimal experimental conditions, the amperometric peak current at 5 s and the subsequent currents at longer test times measured at 0.30 V were linear to the glucose concentration in the range of 0.050–6.0 mg/ml. By constructing a miniature channel on the screen-printed electrode and partially filling the channel with the nanoparticulate sensing membrane, glucose can be accurately determined in as little as 0.20 μl of sample. Successful attempts were made in blood sugar analysis. The results were in good agreement with the reference values obtained with a commercial blood sugar analyzer.