Colloidal gold based electrochemical immunoassays for the diagnosis of acute myocardial infarction

The performed immunoassays are directed to the determination of protein markers for acute myocardial infarction. The determination assays for human myoglobin, cardiac troponin complex and the MB isoform of the enzyme creatine kinase are presented. Cyclic voltammetry measurements are adopted for the determination of colloidal gold used as a label in the immunuassays. The measurements are performed with a screen-printed graphite electrode in a sample volume of 50 μl. The influence of different diameter colloidal gold particles on the assay sensitivity is also investigated.