Direct electron transfer of hemoglobin on PSS/SWNTs film modified Au electrode and its interaction with ribavirin

Direct electrochemistry of hemoglobin (Hb) immobilized on the PSS/SWNTs film modified Au electrode was studied. The immobilized Hb displayed a couple of stable and well-defined redox peaks with the formal potential (E0′) of about 0.117 V (versus SCE) in a 0.1 M phosphate buffer solution (PBS) of pH 7.0. The dependence of E0′ on the solution pH indicated that the direct electron transfer reaction of Hb was that the electrons transfer was accompanied by an equal number of protons in reaction process. Both single-wall carbon nanotubes (SWNTs) and poly(styrene sulfonic acid) sodium salt (PSS) could accelerate the electron transfer between Hb and the electrode. Using the PSS/Hb/SWNTs film modified Au electrode, the interaction between Hb and ribavirin was studied.