Author/Authors :
Atsumi، نويسنده , , Shota and Cann، نويسنده , , Anthony F. and Connor، نويسنده , , Michael R. and Shen، نويسنده , , Claire R. and Smith، نويسنده , , Kevin M. and Brynildsen، نويسنده , , Mark P. and Chou، نويسنده , , Katherine J.Y. and Hanai، نويسنده , , Taizo and Liao، نويسنده , , James C.، نويسنده ,
Abstract :
Compared to ethanol, butanol offers many advantages as a substitute for gasoline because of higher energy content and higher hydrophobicity. Typically, 1-butanol is produced by Clostridium in a mixed-product fermentation. To facilitate strain improvement for specificity and productivity, we engineered a synthetic pathway in Escherichia coli and demonstrated the production of 1-butanol from this non-native user-friendly host. Alternative genes and competing pathway deletions were evaluated for 1-butanol production. Results show promise for using E. coli for 1-butanol production.
