Production of phenylpropanoid amides in recombinant Escherichia coli

Plant-specific phenylpropanoid amides were produced in a recombinant Escherichia coli that expressed 4-coumarate:coenzyme A ligase from Arabidopsis and serotonin N-hydroxycinnamoyltransferase from pepper plants. Upon exogenous treatment with several precursors, high concentrations of the following phenylpropanoid amides were produced abundantly in the culture medium in a few hours: 4-coumaroylserotonin (215 mg/l), 4-coumaroyloctopamine (208 mg/l), and 4-coumaroyltyramine (187 mg/l).