Title of article :
13C metabolic flux analysis for larger scale cultivation using gas chromatography-combustion-isotope ratio mass spectrometry
Author/Authors :
Yuan، نويسنده , , Yongbo and Hoon Yang، نويسنده , , Tae and Heinzle، نويسنده , , Elmar، نويسنده ,
Issue Information :
دوماهنامه با شماره پیاپی سال 2010
Pages :
9
From page :
392
To page :
400
Abstract :
13C-based metabolic flux analysis (13CMFA) is limited to smaller scale experiments due to very high costs of labeled substrates. We measured 13C enrichment in proteinogenic amino acid hydrolyzates using gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS) from a series of parallel batch cultivations of Corynebacterium glutamicum utilizing mixtures of natural glucose and [1-13C] glucose, containing 0%, 0.5%, 1%, 2%, and 10% [1-13C] glucose. Decreasing the [1-13C] glucose content, kinetic isotope effects played an increasing role but could be corrected. From the corrected 13C enrichments in vivo fluxes in the central metabolism were determined by numerical optimization. The obtained flux distribution was very similar to those obtained from parallel labeling experiments using conventional high labeling GC-MS method and to published results. The GC-C-IRMS-based method involving low labeling degree of expensive tracer substrate, e.g. 1%, is well suited for larger laboratory and industrial pilot scale fermentations.
Keywords :
Corynebacterium glutamicum , metabolic flux analysis , GC-C-IRMS , 13C-labeling , Proteinogenic amino acid
Journal title :
Metabolic Engineering
Serial Year :
2010
Journal title :
Metabolic Engineering
Record number :
1429027
Link To Document :
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