Title of article :
Carotenoid-based phenotypic screen of the yeast deletion collection reveals new genes with roles in isoprenoid production
Author/Authors :
?zayd?n، نويسنده , , Bilge and Burd، نويسنده , , Helcio and Lee، نويسنده , , Taek Soon and Keasling، نويسنده , , Jay D.، نويسنده ,
Issue Information :
دوماهنامه با شماره پیاپی سال 2013
Abstract :
Beside their essential cellular functions, isoprenoids have value as pharmaceuticals, nutriceuticals, pesticides, and fuel alternatives. Engineering microorganisms for production of isoprenoids is relatively easy, sustainable, and cost effective in comparison to chemical synthesis or extraction from natural producers. We introduced genes encoding carotenoid biosynthetic enzymes into the haploid yeast deletion collection to identify gene deletions that improved isoprenoid production. Deletions that showed significant improvement in carotenoid production were further screened for production of bisabolene, an isoprenoid alternative to petroleum-derived diesel. Combining those deletions with other mevalonate pathway modifications increased production of bisabolene from 40 mg/L to 800 mg/L in shake-flask cultures. In a fermentation process, this engineered strain produced 5.2 g/L of bisabolene.
Keywords :
carotenoids , biofuel , S. cerevisiae , Bisabolene , Isoprenoids
Journal title :
Metabolic Engineering
Journal title :
Metabolic Engineering