Chemiluminescence imaging assay dipyridamole based on molecular imprinted polymer as recognition material

A simple, sensitive and specific method has been developed for high throughput detection of dipyridamole. The proposed method is based on chemiluminescence (CL) imaging assay combined molecularly imprinted polymer (MIP) recognition. Molecularly imprinted microspheres were prepared using precipitation polymerization with methacrylic acid (MAA) as functional monomer, trimethylolpropane trimethacrylate (TRIM) as the crosslinker and dipyridamole as the template. Polymer as the artificial biomimetic recognition element for dipyridamole was coated in 96-microtiter well plates with poly(vinyl alcohol) (PVA) as glue. The amount of polymer-bound dipyridamole was determination based on dipyridamole peroxyoxalate chemiluminescence (PO-CL) reaction. The emitting light was measured with a high-resolution charge couple device (CCD) camera. Influencing factors were investigated and optimized in detail. The proposed method in this paper exhibits high selectivity and sensitivity to dipyridamole. Under the optimum conditions, the relative CL imaging intensity (AVG) is proportional to the concentration of dipyridamole ranging from 0.02 to 10 μg/ml. The detection limit is 0.006 μg/ml. The method can perform 96 independent measurements simultaneously in 30 min. These results show that the MIP-based CL imaging method can become a useful analytical technology for quick detection of dipyridamole in real sample.