Title of article :
Rapid evaluation of biofilm attachment promoters and biofilm growth orientation using a mini-impedimetric device
Author/Authors :
Goncalves، نويسنده , , Juan Jose and Govind، نويسنده , , Rakesh، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2009
Pages :
8
From page :
341
To page :
348
Abstract :
The time response of the impedance of a thiosulfate oxidizing bacterial strain growing in culture wells containing 10 active gold mini-electrodes was experimentally measured in this study. Based on a proposed RC circuit that describes the electrode-culture well as an equivalent array of capacitors and resistors, the shape of the time response of the observed total capacitance was anticipated to correlate with the preferential biofilm growth direction (perpendicular to or along the gold mini-electrode surface). Based on this correlation and the proposed model, it was found that when pre-coating the electrodes with an aqueous polyethyleneimine (PEI) solution, the spreading of the biofilm was more intense than in the untreated electrodes, which was later observed through confocal laser scanning microscopy. Similar results were obtained when using poly-d-lysine or calf skin collagen as coating agents, though the spreading of the biofilms was always more intense in the PEI coated ones.
Keywords :
Electrical impedance spectroscopy , Biofilm attachment , polyethyleneimine , Model
Journal title :
Sensors and Actuators B: Chemical
Serial Year :
2009
Journal title :
Sensors and Actuators B: Chemical
Record number :
1437968
Link To Document :
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