Author/Authors :
McGuinness، نويسنده , , Colin D. and Keszenman-Pereyra، نويسنده , , David and Dickinson، نويسنده , , Paul S. Campbell، نويسنده , , Colin J. and Maltman، نويسنده , , Beatrice A. and Bachmann، نويسنده , , Till T. and Ghazal، نويسنده , , Peter and Crain، نويسنده , , Jason، نويسنده ,
Abstract :
Detection of single base pair mutations in an unlabelled nucleic acid target sequence is demonstrated using the time-resolved measurement of the Fِrster Resonance Energy Transfer (FRET) between a long lifetime acridine-based fluorophore and a non-fluorescent quencher molecule covalently bound to a DNA Holliday junction-based nanoswitch.
Keywords :
FRET , Single photon counting , DNA , holliday junction , fluorescence
