Title of article :
Development of a high sensitive automatic setup for screening of microcystins in surface waters by employing a LED-based photometric detector
Author/Authors :
Vieira، نويسنده , , Glلucia P. and Perdigمo، نويسنده , , Sheila R.W. and Fiore، نويسنده , , Marli F. and Reis، نويسنده , , Boaventura F. Reis، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2012
Pages :
7
From page :
422
To page :
428
Abstract :
In this manuscript, an automatic setup for screening of microcystins in surface waters by employing photometric detection is described. Microcystins are toxins delivered by cyanobacteria within an aquatic environment, which have been considered strongly poisonous for humans. For that reason, the World Health Organization (WHO) has proposed a provisional guideline value for drinking water of 1 μg L−1. In this work, we developed an automated equipment setup, which allows the screening of water for concentration of microcystins below 0.1 μg L−1. The photometric method was based on the enzyme-linked immunosorbent assay (ELISA) and the analytical signal was monitored at 458 nm using a homemade LED-based photometer. The proposed system was employed for the detection of microcystins in rivers and lakes waters. Accuracy was assessed by processing samples using a reference method and applying the paired t-test between results. No significant difference at the 95% confidence level was observed. Other useful features including a linear response ranging from 0.05 up to 2.00 μg L−1 (R2 = 0.999) and a detection limit of 0.03 μg L−1 microcystins were achieved.
Keywords :
Microcystins , surface waters , Enzyme-linked immunosorbent assay , Automation , LED-based photometer , Multicommuted flow analysis
Journal title :
Sensors and Actuators B: Chemical
Serial Year :
2012
Journal title :
Sensors and Actuators B: Chemical
Record number :
1440175
Link To Document :
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