Title of article :
Dual amplified, sensitive electrochemical detection of pathogenic sequences based on biobarcode labels and functional graphene modified electrode
Author/Authors :
Wang، نويسنده , , Qiong and Su، نويسنده , , Jiao and Xu، نويسنده , , Jin and Xiang، نويسنده , , Yun and Yuan، نويسنده , , Ruo and Chai، نويسنده , , Yaqin، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2012
Pages :
5
From page :
267
To page :
271
Abstract :
A dual signal amplified strategy for sensitive EC detection of pathogenic DNA sequences based on poly[G]20-conjugated biobarcode nanoparticles and carboxyl functionalized graphene is described. The capture probes are covalently linked to the graphene modified electrode. The addition of the target sequences and the biobarcode amplification labels leads to the formation of sandwich structures on the electrode surface. The presence of Ru(bpy)32+ in the detection buffer catalyzes the electro-oxidation of the numerous guanine bases captured on the electrode surface and generates significantly amplified current response for quantitation. Moreover, graphene can offer excellent heterogeneous electron transfer during electro-catalytic oxidation of guanine bases. This dual signal amplification strategy thus results in the determination of pathogenic DNA sequences down to the low picomolar level (1 pM). Our approach also exhibits excellent discrimination between the target and single-base mismatch sequences, which provides great potential for highly sensitive and selective detection of different nucleic acid sequences in general.
Keywords :
Amplification , Biobarcode , Pathogen detection , graphene
Journal title :
Sensors and Actuators B: Chemical
Serial Year :
2012
Journal title :
Sensors and Actuators B: Chemical
Record number :
1440462
Link To Document :
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