Title of article
Colorimetric detection of cancer biomarker based on pH induced color change
Author/Authors
Chu، نويسنده , , Binyang and Qi، نويسنده , , Tingting and Liao، نويسنده , , Jinfeng and Peng، نويسنده , , Jingrong and Li، نويسنده , , Wenting and Fu، نويسنده , , Shaozhi and Luo، نويسنده , , Wei Feng and Qian Huang، نويسنده , , Zhiyong، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2012
Pages
5
From page
56
To page
60
Abstract
A simple, easy, colorimetric immunoassay for cancer biomarker prostate specific antigen (PSA) is reported based on pH induced color change. Glutathione functionalized gold nanoparticles (Glu-AuNPs) were prepared and used as label for the immunoassay, while dopamine anchored Fe3O4 (DA-Fe3O4) was selected to immobilize anti-PSA antibody (Ab1). AuNPs and Fe3O4 were further assembled into immuno-complex in the presence of PSA. After the separation of the immuno-complex using magnet, the left AuNPs was mixed with methyl red solution and displaying colors. Different colors corresponding to different concentrations of PSA can be directly detected with eyes. This novel immunoassay has great potential to the detection of other cancer biomarkers.
Keywords
Colorimetric Assay , Immuno-complex , Prostate Specific Antigen , Cancer biomarker , Immunoassay
Journal title
Sensors and Actuators B: Chemical
Serial Year
2012
Journal title
Sensors and Actuators B: Chemical
Record number
1440515
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