Author/Authors :
Huang، نويسنده , , Xijing and Hao، نويسنده , , Yanlin and Wu، نويسنده , , Hanyin and Guo، نويسنده , , Qingquan and Guo، نويسنده , , Liangqia and Wang، نويسنده , , Jing and Zhong، نويسنده , , Liangshuang and Lin، نويسنده , , Tianran and Fu، نويسنده , , FengFu and Chen، نويسنده , , Guonan، نويسنده ,
Abstract :
A simple and sensitive DNAzyme-based colorimetric sensor for detecting Hg2+ based on T–Hg2+-T base pair and magnetic beads (MBs) is proposed. In the presence of Hg2+, capture probes (CPs) immobilized on MBs are hybridized with the signal probes (SPs) consisting of G-quadruplex sequences with the help of T–Hg2+-T base pairs. The SPs are separated by MBs and used to bind hemin for the formation of catalytically active hemin/G-quadruplex DNAzymes, which catalyzes the H2O2-mediated oxidation of ABTS to produce a blue-green-colored product. A linear relationship between the absorbance and Hg2+ concentration is observed over a range of 10–200 nM with a limit of detection 5 nM. Taking advantage of MBs separation/collection process, the sensing system can be regenerated by using EDTA as the chelator.
Keywords :
T–Hg2+-T , Magnetic beads , DNAzyme , mercury
