Author/Authors :
Singh، نويسنده , , Chandan and Srivastava، نويسنده , , Saurabh and Ali، نويسنده , , Md. Azahar and Gupta، نويسنده , , Tejendra K. and Sumana، نويسنده , , Gajjala and Srivastava، نويسنده , , Anchal and Mathur، نويسنده , , R.B. and Malhotra، نويسنده , , Bansi D. Malhotra، نويسنده ,
Abstract :
We report results of studies relating to the development of an electrochemical immunosensor based on carboxylated multiwalled carbon nanotubes (c-MWCNTs) electrophoretically deposited onto indium tin oxide (ITO) glass. This c-MWCNTs/ITO electrode surface has been functionalized with monoclonal aflatoxin B1 antibodies (anti-AFB1) for the detection of aflatoxin-B1 using electrochemical technique. Electron microscopy, X-ray diffraction and Raman studies suggest successful synthesis of c-MWCNTs and the Fourier transform infra-red spectroscopic (FT-IR) studies reveal its carboxylic functionalized nature. The proposed immunosensor shows high sensitivity (95.2 μA ng−1 mL cm−2), improved detection limit (0.08 ng mL−1) in the linear detection range of 0.25–1.375 ng mL−1. The low value of association constant (0.0915 ng mL−1) indicates high affinity of immunoelectrode towards aflatoxin (AFB1).
Keywords :
Aflatoxin B1 , Multiwalled carbon nanotubes , Electrochemical detection , Electrophoretic Deposition
