Author/Authors :
Chen، نويسنده , , L.H. and Chan، نويسنده , , C.C. and Menon، نويسنده , , R. and Balamurali، نويسنده , , P. and Wong، نويسنده , , W.C. and Ang، نويسنده , , X.M. and Hu، نويسنده , , P.B. and Shaillender، نويسنده , , M. P. Neu، نويسنده , , B. and Zu، نويسنده , , P. and Tou، نويسنده , , Z.Q. and Poh، نويسنده , , C.L. and Leong، نويسنده , , K.C.، نويسنده ,
Abstract :
A label-free fiber-optic Fabry–Perot interferometric (FPI) immunosensor is proposed by layer-by-layer (LBL) self-assembly of a suspended chitosan and polystyrene sulfonate membrane at the tip of a hollow fiber. Immunoglobulin G (IgG) was immobilized on the polyelectrolyte substrate and the detection of target anti-immunoglobulin G (anti-IgG) was monitored through the measurement of substrateʹs effective optical thickness. The sensitivity and specificity of the sensor were studied using correlated and non-correlated anti-IgG/IgG pair. Sensitivity of 0.033 μm/(pg/mm2) and limit of detection of 0.005 nM was achieved with the sensor displaying minimal response toward non-correlated anti-IgG. The results demonstrate the feasibility of the sensor for immunosensing.
Keywords :
Fabry–Perot interferometer , Layer-by-layer self assembly , Biosensor , Immunosensor
