Title of article :
Effects of anti-GLUT antibodies on glucose transport into human erythrocyte ghosts
Author/Authors :
Afzal، نويسنده , , I. and Browning، نويسنده , , J.A. and Drew، نويسنده , , C. and Ellory، نويسنده , , J.C. and Naftalin، نويسنده , , R.J. and Wilkins، نويسنده , , R.J.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2004
Pages :
4
From page :
195
To page :
198
Abstract :
We have studied the effects of anti-GLUT1 antibodies on the uptake of glucose into erythrocytes. Glucose transport into human erythrocyte ghosts was measured directly using 3H-2-deoxy-glucose, or indirectly by monitoring associated volume changes using light scattering. The uptake of glucose was significantly inhibited in ghosts resealed in solutions containing specific antibodies against GLUT1. Such an effect was not observed when an antibody against the oestrogen receptor, lacking specificity towards GLUT1, was employed instead. The antibodies were also without effect on the efflux of preloaded glucose from erythrocyte ghosts. The demonstration that anti-GLUT antibodies can inhibit glucose uptake is support for the hypothesis that they exaggerate the cytoplasmic barrier to glucose uptake created by endofacial segments of GLUT1.
Keywords :
GLUT1 , erythrocyte , ghost , antibody
Journal title :
Bioelectrochemistry
Serial Year :
2004
Journal title :
Bioelectrochemistry
Record number :
1450859
Link To Document :
بازگشت