Real-time assay of immobilized tannase with a stopped-flow conductometric device

A stopped-flow manifold was developed to assay and characterize immobilized tannase (EC 3.1.1.20). The immobilized enzyme reactor was inserted within the tube-type electrode pair (cell constant = 103.2 cm− 1) for a real-time conductometric measurement. Tris buffer (2 mM, pH = 7.0) was used as the carrier for sensitivity improvement. The activities and kinetic parameters (Km values) for propyl gallate, methyl gallate and tannic acid were investigated.