Title of article :
Electrochemical probe DNA design in PCR amplicon sequence for the optimum detection of microbiological diseases
Author/Authors :
Kara، نويسنده , , Pinar and Cavdar، نويسنده , , Seda and Meric، نويسنده , , Buket and Erensoy، نويسنده , , Selda and Ozsoz، نويسنده , , Mehmet، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2007
Pages :
7
From page :
204
To page :
210
Abstract :
Direct electrochemical genosensor was developed for the detection of a probe sequence relative position in a PCR amplicon for the optimum detection of bacterial and microbiological diseases, in this study. The genosensor relies on a label-free electrochemical detection. The amino-linked inosine modified (guanine-free) coequal capture probes which were chosen from different parts of a PCR amplicon, immobilized on to disposable pencil graphite electrodes (PGE) by electrostatically and covalently. As a model case Hepatitis B virus (HBV) genome amplicon was used for the detection and specification. Hybridization was occurred after surface coverage with denatured amplicons. After hybridization, optimum probe sequence position was identified by using the differences between the responses of guanine oxidation signals. The results of this study might have a great convenience for the microbiological diseases detection applications such as DNA micro arrays.
Keywords :
Electrochemical genosensor , DNA , PCR
Journal title :
Bioelectrochemistry
Serial Year :
2007
Journal title :
Bioelectrochemistry
Record number :
1451810
Link To Document :
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