Electrochemical and Ultraviolet–visible spectroscopic studies on the interaction of deoxyribonucleic acid with vitamin B6

Studies on the interaction of DNA with vitamin B6 were carried out with a DNA-modified electrode by electrochemistry and Ultraviolet–visible spectroscopy. The results showed that there exists the supra-molecule interaction between base groups on DNA and vitamin B6 by forming hydrogen binding, the binding equilibrium constant of the interaction is equal to 115.3 M−1, the binding ratio of nucleotide to vitamin B6 is 5:1. Based on the electrochemical and Ultraviolet–visible spectrum studies the interaction mode of DNA with vitamin B6 was explored.