Evaluation of human hepatocyte incubation as a new tool for metabolism study of androstenedione and norandrostenedione in a doping control perspective

Human hepatocyte incubations were used to study the metabolism of precursors of testosterone and nortestosterone and to evaluate qualitatively the correlation between in vitro and published in vivo urinary metabolic profiles. Both phase I and phase II biotransformations were observed in vitro: oxidoreduction at C-3 and C-17, reduction at C-4,5, hydroxylation at C-6β and C-16, glucuronidation and sulfation. All major metabolites detected in urine following oral administration of androstenedione and norandrostenedione were present in human hepatocyte incubations. The good correlation between in vitro and in vivo metabolic profiles indicates that hepatocyte incubations can be a useful tool to identify and characterize metabolites that could be potential urinary markers for detection of steroid abuse by athletes.