Quantitation of yeast ceramides using high-performance liquid chromatography–evaporative light-scattering detection

A high-performance liquid chromatograph equipped with an evaporative light scattering detector (ELSD) (HPLC–ELSD) was used to assay the ceramides in yeast cells. The HPLC–ELSD method employed a cyanopropyl bonded column (CN column) that effectively separated the main interfering substance ergosterol without any derivatization process; most other interfering substances were also removed. The method can be applied for routine assay of ceramide content in yeast.