Author/Authors :
Keinنnen، نويسنده , , M.M. and Korhonen، نويسنده , , L.K. and Martikainen، نويسنده , , P.J. and Vartiainen، نويسنده , , T. A. Miettinen، نويسنده , , I.T. and Lehtola، نويسنده , , M.J. and Nenonen، نويسنده , , K. and Pajunen، نويسنده , , H. and Kontro، نويسنده , , M.H.، نويسنده ,
Abstract :
Hydroxy fatty acids (OH-FAs) can be used in the characterization of microbial communities, especially Gram-negative bacteria. We prepared methyl esters of 2- and 3-OH-FAs from the lipid extraction residue of soil, sediment, and biofilm samples without further purification or derivatization of hydroxyl groups. OH-FA methyl esters were analyzed using a gas chromatograph equipped with a mass selective detector (GC–MS). The ions followed in MS were m/z 103 for 3-OH-FAs and m/z 90 and M-59 for 2-OH-FAs. The rapid determination of 3- and 2-OH-FAs concomitantly with phospholipid fatty acids provided more detailed information on the microbial communities present in soil, sediment, and drinking water biofilm.
