Author/Authors :
Accinni، نويسنده , , Roberto and Campolo، نويسنده , , Jonica and Parolini، نويسنده , , Marina and De Maria، نويسنده , , Renata and Caruso، نويسنده , , Raffaele and Maiorana، نويسنده , , Andrea and Galluzzo، نويسنده , , Cino and Bartesaghi، نويسنده , , Silvia and Melotti، نويسنده , , Davide and Parodi، نويسنده , , Oberdan، نويسنده ,
Abstract :
Identification of homocystinuric newborns is hindered by the pitfalls of neonatal screening programs. We propose a fluorimetric HPLC method with a rapid pre-analytical step for homocysteine determination from neonatal dried blood spot cards. Homocysteine in blood spots sampled among 2000 healthy newborns on living day 4, averaged 2.92±2.07 μM (range 0.4–7.5). In eight homocystinuric control children, mean values were 61.71±52.84 μM (range 18.9–145.7). The method showed a good linearity (r=0.999), precision (RSD<7%) and recovery (95%). The correlation between blood spots and plasma samples was r=0.90. This method has all the essential features for a homocystinuria screening program: an easy and rapid pre-analytical step combined with method linearity and precision.
